Translocation of antibiotics in plants. (1) Effect of plant tissue on the assays of some antibiotics and translocation of them in broad bean.

Tomomasa MISATO; Masaru ASAKAWA; Kazuo FUKUNAGA

doi:10.3186/jjphytopath.23.97

Translocation of antibiotics in plants.

(1) Effect of plant tissue on the assays of some antibiotics and translocation of them in broad bean.

Tomomasa MISATO, Masaru ASAKAWA, Kazuo FUKUNAGA

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JOURNAL FREE ACCESS

1958 Volume 23 Issue 2 Pages 97-101

DOI https://doi.org/10.3186/jjphytopath.23.97

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Published: 1958 Received: - Available on J-STAGE: February 19, 2009 Accepted: April 16, 1958 Advance online publication: - Revised: -
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Date of correction: February 19, 2009 Reason for correction: - Correction: TITLE Details: Wrong : Translocation of antibiotics in plants
Right : Translocation of antibiotics in plants.

Date of correction: February 19, 2009 Reason for correction: - Correction: SUBTITLE Details: Wrong : (1) Effect of plant tissue on the assays of some antibiotics and translocation of them in broad bean
Right : (1) Effect of plant tissue on the assays of some antibiotics and translocation of them in broad bean.

Date of correction: February 19, 2009 Reason for correction: - Correction: ABSTRACT Details: Wrong : (1) By assaying on a 4-ml agar plate (soluble starch 1%, yeast extract powder 0.1%, agar 1.5%) seeded with a spore suspension of Piricularia oryzae, Antimycin A could be detected at a concentration of 0.01mcg/ml, Blasticidin at 0.05mcg/ml and Blastmycin at 0.003mcg/ml. Antimycin A and Blastmycin were assayed by the paperdisc method and Blasticidin by the cup method.
(2) Antimycin A and Blastmycin in plants were extracted by methanol and Blasticidin by the pH 5.0 buffer solution. The juice of leaves of untreated tomato had antibiotic activity against P. oryzae, and conversely that of rice or broad bean promoted its growth. Methanol extracts of leaves of untreated tomato, rice or broad bean gave inhibition zones. Therefore the buffer or methanol extracts of leaves were assayed, after being diluted with buffer or methanol to the concentration in which the plant tissue had no effect upon P. oryzae.
(3) Zone sizes of Blasticidin were smaller in broad bean leaf extract than in the buffer solution; but they were about the same in rice or tomato extract as in the buffer solution. Approximately 80 of the antifungal activity of Blasticidin decreased in broad bean leaf extract. Zone sizes of Antimycin A and Blastmycin were smaller in rice leaf extract than in methanol, but about the same in tomato or broad bean extract as methanol. Approximately 70% of the antifungal activity of Antimycin A and Blastmycin decreased in rice leaf extract.
(4) When the cut stems of broad bean were placed in solutions containing 5∼10mcg/ml of Antimycin A, Blasticidin and Blastmycin, antibiotics were absorbed and translocated upward to leaves. Leaves were found to contain only 0.1∼0.3mcg/g of Antimycin A or Blastmycin, or 3.0∼6.0mcg/g of Blasticidin after several days. But no antibiotics were detected in leaves of root-treated plants.
Right : (1) By assaying on a 4-ml agar plate(soluble starch 1%, yeast extract powder 0.1%, agar 1.5 %) seeded with a spore suspension of Piricularia oryzae, Antimycin A could be detected at a concentration of 0.01mcg/ml, Blasticidin at 0.05 mcg/ml and Blastmycin at 0.003mcg/ml. Antimycin A and Blastmycin were assayed by the paperdisc method and Blasticidin by the cup method.
(2) Antimycin A and Blastmycin in plants were extracted by methanol and Blasticidin by the pH 5.0 buffer solution. The juice of leaves of untreated tomato had antibiotic activity against P. oryzae, and conversely that of rice or broad bean promoted its growth. Methanol extracts of leaves of untreated tomato, rice or broad bean gave inhibition zones. Therefore the buffer or methanol extracts of leaves were assayed, after being diluted with buffer or methanol to the concentration in which the plant tissue had no effect upon P. oryzae.
(3) Zone sizes of Blasticidin were smaller in broad bean leaf extract than in the buffer solution ; but they were about the same in rice or tomato extract as in the buffer solution. Approximately 80 % of the antifungal activity of Blasticidin decreased in broad bean leaf extract. Zone sizes of Antimycin A and Blastmycin were smaller in rice leaf extract than in methanol, but about the same in tomato or broad bean extract as methanol. Approximately 70 % of the antifungal activity of Antimycin A and Blastmycin decreased in rice leaf extract.
(4) When the cut stems of broad bean were placed in solutions containing 5-10mcg/ml of Antimycin A, Blasticidin and Blastmycin, antibiotics were absorbed and translocated upward to leaves. Leaves were found to contain only 0.1-0.3mcg/g of Antimycin A or Blastmycin, or 3.0-6.0mcg/g of Blasticidin after several days. But no antibiotics were detected in leaves of root-treated plants.

Date of correction: February 19, 2009 Reason for correction: - Correction: DTPUB Details: Wrong : 19580730

Date of correction: February 19, 2009 Reason for correction: - Correction: AUTHOR Details: Wrong : Tomomasa MISATO, Masaru ASAKAWA, Kazuo FUKUNAGA¹⁾
Right : Tomomasa MISATO¹⁾, Masaru ASAKAWA¹⁾, Kazuo FUKUNAGA¹⁾

Date of correction: February 19, 2009 Reason for correction: - Correction: CITATION Details: Wrong : 1) Blanchard, F. A.: Phytopath., 41: 955-958. (1951),.
2) Gopalkrishnan, K. S. and Jump, J. A.: Phytopath., 42: 338-339. (1952).
4) Mitchell, J. W., Zaumeyer, W. J. and Preston, W. H.: Phytopath., 44: 25-30. (1954).
5) Mitchell, J. W., Zaumeyer, W. J. and Anderson, W. P.: Science, 115: 114-115. (1952).
6) Hamilton, J. M., Szkolnik, M. and Sondheimer, E.: Science, 123: 1175-1176. (1956).
7) Leben, C. and Fulton, R. W: Phytopath., 42: 331-335. (1952).
8) Dye, D. W.: Nature, 172: 683. (1953).
10) Pramer, D.: Ann. Appl. Biol., 40: 617-622. (1953).
11) Crowdy, S. H. and Pramer, D.: Chem. & Ind. (Rev.), 7: 160-162. (1955).
12) Blnachard. F. A. and Diller, V. M.: Amer. J. Bot., 38: 111-112. (1951).
13) Gray, R. A.: Phytopath., 48: 71-78. (1958).
14) Pramer, D.: Ann. Bot., 18 (72): 463-470. (1954).
15) Crowdy, S. H. and Pramer, D.: Ann. Bot., 19 (73): 79-86. (1955).
16) Nakayama, K., et al.: J. Antibotics, Ser. A. 9(2): 63-66. (1956).
17) Fukunaga, K., et al.: Bull. Agri. Chem. Japan, 19: 181-188. (1955).
18) Watanabe, K., et al.: J. Antibiotics, Ser. A. 10: 39-45. (1957).
20) Dye, M. H.: Ann. Appl. Biol., 44: 567-575. (1956).
22) Food u. Drug Administ: Federal Register April, 4. (1947).
24) Leben, C. and Keitt, G. W.: Phytopath., 39: 529-540. (1949).
25) Wallen, V. R. and Miller, R. L.: Phytopath., 47: 291-294. (1957).
26) Brian, P. W.: Ann. Appl. Biol., 39: 434-438. (1952).
27) Stokes, A.: Plant & Soil, 5 (2): 132-142. (1954).
28) Stubbs, J.: Ann. Appl. Biol., 39: 439-441. (1952).

Right : 1) Blanchard, F.A.: Phytopath., 41: 955-958. (1951),.
2) Gopalkrishnan, K.S. and Jump, J.A.: Phytopath., 42: 338-339. (1952).
4) Mitchell, J.W., Zaumeyer, W.J. and Preston, W.H.: Phytopath., 44: 25-30. (1954).
5) Mitchell, J.W., Zaumeyer, W.J. and Anderson, W.P.: Science, 115: 114-115. (1952).
6) Hamilton, J.M., Szkolnik, M. and Sondheimer, E.: Science, 123: 1175-1176, (1956).
7) Leben, C. and Fulton, R. W: Phytopath., 42: 331-335. (1952).
8) Dye, D.W.: Nature, 172: 683. (1953).
10) Pramer, D.: Ann. Appl. Biol., 40: 617-622. (1953).
11) Crowdy, S.H. and Pramer, D.: Chem. & Ind. (Rev.), 7: 160-162.(1955).
12) Blnachard. F.A. and Diller, V.M.: Amer. J. Bot., 38: 111-112. (1951).
13) Gray, R.A.: Phytopath., 48: 71-78. (1958).
14) Pramer, D.: Ann. Bot., 18 (72): 463-470. (1954).
15) Crowdy, S.H. and Pramer, D.: Ann. Bot., 19 (73): 79-86. (1955).
16) Nakayama, K., et al.: J. Antibotics, Ser. A. 9 (2): 63-66. (1956).
17) Fukunaga, K., et al.: Bull. Agri. Chem. Japan, 19: 181-188. (1955).
18) Watanabe, K., et al.: J. Antibiotics, Ser. A. 10: 39-45. (1957).
20) Dye, M.H.: Ann. Appl. Biol., 44: 567-575. (1956).
22) Food u. Drug Administ: Federal Register April, 4. (1947).
24) Leben, C. and Keitt, G.W.: Phytopath., 39: 529-540. (1949).
25) Wallen, V.R. and Miller, R.L.: Phytopath., 47: 291-294. (1957).
26) Brian, P.W.: Ann. Appl. Biol., 39: 434-438. (1952).
27) Stokes, A.: Plant & Soil, 5 (2): 132-142. (1954).
28) Stubbs, J.: Ann, Appl. Biol., 39: 439-441 (1952).

Date of correction: February 19, 2009 Reason for correction: - Correction: PDF FILE Details: -

Abstract

(1) By assaying on a 4-ml agar plate(soluble starch 1%, yeast extract powder 0.1%, agar 1.5 %) seeded with a spore suspension of Piricularia oryzae, Antimycin A could be detected at a concentration of 0.01mcg/ml, Blasticidin at 0.05 mcg/ml and Blastmycin at 0.003mcg/ml. Antimycin A and Blastmycin were assayed by the paperdisc method and Blasticidin by the cup method.
(2) Antimycin A and Blastmycin in plants were extracted by methanol and Blasticidin by the pH 5.0 buffer solution. The juice of leaves of untreated tomato had antibiotic activity against P. oryzae, and conversely that of rice or broad bean promoted its growth. Methanol extracts of leaves of untreated tomato, rice or broad bean gave inhibition zones. Therefore the buffer or methanol extracts of leaves were assayed, after being diluted with buffer or methanol to the concentration in which the plant tissue had no effect upon P. oryzae.
(3) Zone sizes of Blasticidin were smaller in broad bean leaf extract than in the buffer solution ; but they were about the same in rice or tomato extract as in the buffer solution. Approximately 80 % of the antifungal activity of Blasticidin decreased in broad bean leaf extract. Zone sizes of Antimycin A and Blastmycin were smaller in rice leaf extract than in methanol, but about the same in tomato or broad bean extract as methanol. Approximately 70 % of the antifungal activity of Antimycin A and Blastmycin decreased in rice leaf extract.
(4) When the cut stems of broad bean were placed in solutions containing 5-10mcg/ml of Antimycin A, Blasticidin and Blastmycin, antibiotics were absorbed and translocated upward to leaves. Leaves were found to contain only 0.1-0.3mcg/g of Antimycin A or Blastmycin, or 3.0-6.0mcg/g of Blasticidin after several days. But no antibiotics were detected in leaves of root-treated plants.

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