Biochemical studies on Cochliobolus miyabeanus. III. Some oxidizing enzymes of the rice plant and its parasites and their contribution to the formation of the lesions.

Hachiro OKU

doi:10.3186/jjphytopath.23.169

Biochemical studies on Cochliobolus miyabeanus. III.

Some oxidizing enzymes of the rice plant and its parasites and their contribution to the formation of the lesions.

Hachiro OKU

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JOURNAL FREE ACCESS

1958 Volume 23 Issue 4 Pages 169-175

DOI https://doi.org/10.3186/jjphytopath.23.169

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Published: November 30, 1958 Received: - Available on J-STAGE: February 19, 2009 Accepted: October 13, 1958 Advance online publication: - Revised: -
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Date of correction: February 19, 2009 Reason for correction: - Correction: TITLE Details: Wrong : Biochemical studies on Cochliobolus miyabeanus. III
Right : Biochemical studies on Cochliobolus miyabeanus. III.

Date of correction: February 19, 2009 Reason for correction: - Correction: SUBTITLE Details: Wrong : Some oxidizing enzymes of the rice plant and its parasites and their contribution to the formation of the lesions
Right : Some oxidizing enzymes of the rice plant and its parasites and their contribution to the formation of the lesions.

Date of correction: February 19, 2009 Reason for correction: - Correction: ABSTRACT Details: Wrong : Some oxidizing enzymes of Oryza sativa (rice plant), Cochliobolus miyabeanus, and Piricularia oryzae were investigated.
(1) Presence of cytochrome oxidase was suggested in O. sativa, C. miyabeanus, and P. oryzae from the fact that the oxygen uptake was increased by addition of cytocrome-c into the hydroquinone-enzyme system, or into the p-phenylenediamine-enzyme system. High ascorbic acid oxidase activity was also found.
(2) High polyphenol oxidase activity was found in C. miyabeanus and various plant polyphenols were oxidized by the same enzyme. The pH optimum of the enzyme was found to be 8.0. The enzyme was inhibited by diethyldithiocarbamate or KCN. However, polyphenol oxidase activity was very low in O. sativa and P. oryzae.
(3) Growth of C. miyabeanus was inhibited and brown pigmentation was observed when cultured on the media containing various polyphenols, namely chlorogenic acid, crude polyphenol mixture extracted from rice plant, and so on.
(4) From the above results, polyphenol oxidase of the causal fungus seemed to play an important role on the appearance of the symptom, “brown spot formation”, of leaf blight disease in the rice plant.
Right : Some oxidizing enzymes of Oryza sativa (rice plant), Cochliobolus miyabeanus, and Piricularia oryzae were investigated.
(1) Presence of cytochrome oxidase was suggested in O. sativa, C. miyabeanus, and P. oryzae from the fact that the oxygen uptake was increased by addition of cytocrome-c into the hydroquinone-enzyme system, or into the p-phenylenediamine-enzyme system. High ascorbic acid oxidase activity was also found.
(2) High polyphenol oxidase activity was found in C. miyabeanus and various plant polyphenols were oxidized by the same enzyme. The pH optimum of the enzyme was found to be 8.0. The enzyme was inhibited by diethyldithiocarbamate or KCN. However, polyphenol oxidase activity was very low in O. sativa and P. oryzae.
(3) Growth of C. miyabeanus was inhibited and brown pigmentation was observed when cultured on the media containing various polyphenols, namely chlorogenic acid, crude polyphenol mixture extracted from rice plant, and so on.
(4) From the above results, polyphenol oxidase of the causal fungus seemed to play an important role on the appearance of the symptom, “brown spot formation”, of leaf blight disease in the rice plant.

Date of correction: February 19, 2009 Reason for correction: - Correction: CITATION Details: Wrong : 2) Daly, J. M. and R. M. Sayre: Phytopath., 47: 163-168. (1957).
3) Daly, J. M., R. M. Sayre & J. H. Pazur: Plant Physiol., 32: 44-48. (1957).
4) Kiraly, Z. and G. L. Farkas: Phytopath., 47: 277-278. (1957).
5) -·-: Arch. Biochem. Biophys., 66: 474-485. (1957).
6) Hill, R. and E. F. Hartree: Ann. Rev. Plant Physiol., 4: 115-150. (1953).
8) Rudkin, G. O. and J. M. Nelson: J. Am. Chem. Soc., 69: 1470-1475. (1947).
15) Uritani, I. and T. Akazawa: Science, 121: 216. (1955).

Right : 2) Daly, J.M. and R.M. Sayre: Phytopath., 47: 163-168. (1957).
3) Daly, J.M. and R.M. Sayre & J.H. Pazur: Plant Physiol., 32: 44-48. (1957).
4) Kiraly, Z. and G.L. Farkas: Phytopath., 47: 277-278. (1957).
5) Kiraly, Z.·G.L. Farkas: Arch. Biochem. Biophys., 66: 474-485. (1957).
6) Hill, R. and E.F. Hartree: Ann. Rev. Plant Physiol., 4: 115-150. (1953).
8) Rudkin, G.O. and J.M. Nelson: J. Am. Chem. Soc., 69: 1470-1475. (1947).
15) Uritani, I. and T. Akazawa: Science, 121: 216. (1955).

Date of correction: February 19, 2009 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Some oxidizing enzymes of Oryza sativa (rice plant), Cochliobolus miyabeanus, and Piricularia oryzae were investigated.
(1) Presence of cytochrome oxidase was suggested in O. sativa, C. miyabeanus, and P. oryzae from the fact that the oxygen uptake was increased by addition of cytocrome-c into the hydroquinone-enzyme system, or into the p-phenylenediamine-enzyme system. High ascorbic acid oxidase activity was also found.
(2) High polyphenol oxidase activity was found in C. miyabeanus and various plant polyphenols were oxidized by the same enzyme. The pH optimum of the enzyme was found to be 8.0. The enzyme was inhibited by diethyldithiocarbamate or KCN. However, polyphenol oxidase activity was very low in O. sativa and P. oryzae.
(3) Growth of C. miyabeanus was inhibited and brown pigmentation was observed when cultured on the media containing various polyphenols, namely chlorogenic acid, crude polyphenol mixture extracted from rice plant, and so on.
(4) From the above results, polyphenol oxidase of the causal fungus seemed to play an important role on the appearance of the symptom, “brown spot formation”, of leaf blight disease in the rice plant.

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