On the Phytoalexin production of the soybean pod in reaction to Fusarium sp., the causal fungus of pod blight. I. Some experiments on the Phytoalexin production as affected by host plant conditions and on the nature of Phytoalexin produced.

Kazuo UEHARA

doi:10.3186/jjphytopath.23.225

On the Phytoalexin production of the soybean pod in reaction to Fusarium sp., the causal fungus of pod blight.

I. Some experiments on the Phytoalexin production as affected by host plant conditions and on the nature of Phytoalexin produced.

Kazuo UEHARA

Author information

JOURNAL FREE ACCESS

1958 Volume 23 Issue 5 Pages 225-229

DOI https://doi.org/10.3186/jjphytopath.23.225

Details

Published: December 30, 1958 Received: - Available on J-STAGE: February 19, 2009 Accepted: February 12, 1958 Advance online publication: - Revised: -
Correction information
Date of correction: February 19, 2009 Reason for correction: - Correction: TITLE Details: Wrong : On the Phytoalexin production of the soybean pod in reaction to Fusarium sp., the causal fungus of pod blight
Right : On the Phytoalexin production of the soybean pod in reaction to Fusarium sp., the causal fungus of pod blight.

Date of correction: February 19, 2009 Reason for correction: - Correction: SUBTITLE Details: Wrong : I. Some experiments on the Phytoalexin production as affected by host plant conditions and on the nature of Phytoalexin produced
Right : I. Some experiments on the Phytoalexin production as affected by host plant conditions and on the nature of Phytoalexin produced.

Date of correction: February 19, 2009 Reason for correction: - Correction: ABSTRACT Details: Wrong : Phytoalexin (PA for short) has been defined by Müller (1954) as antibiotics which are the result of an interaction between the host and the parasite, and which inhibit the growth of microorganism pathogenic to plants. In the present paper the results are dealt, of a study on the rate of production of PA, as affected by host plant conditions, and activities of PA, using soybean (Gylcine max Mer.) and Fusarium sp. which causes pod blight.
Drops of spore suspension of Fusarium were mounted on the inner surface of the seed rooms of the pods at different ages, and after centrifuging, the supernatant was tested for inhibitory action on the germination of fresh spores of Fusarium. It was found that the PA diffused distinctly into the drops placed on the young, unmatured pods, while hardly or not in the drops on the mature pods.
The PA productivity of the host tissue was determined by changing daily the drops placed on given points of young pods with new spore suspension, and by measuring the inhibitory action of the diffusates on each day. It was shown that the production of PA decreased remarkably on the second day, and sank nearly to zero on the fourth day.
PA in the diffusate from the pods lost most of its inhibitory activity, when heated at 100°C for 5 minutes or diluted to 1:8.
Right : Phytoalexin (PA for short) has been defined by Müller (1954) as antibiotics which are the result of an interaction between the host and the parasite, and which inhibit the growth of microorganism pathogenic to plants. In the present paper the results are dealt, of a study on the rate of production of PA, as affected by host plant conditions, and activities of PA, using soybean (Gylcine max Mer.) and Fusarium sp. which causes pod blight.
Drops of spore suspension of Fusarium were mounted on the inner surface of the seed rooms of the pods at different ages, and after centrifuging, the supernatant was tested for inhibitory action on the germination of fresh spores of Fusarium. It was found that the PA diffused distinctly into the drops placed on the young, unmatured pods, while hardly or not in the drops on the mature pods.
The PA productivity of the host tissue was determined by changing daily the drops placed on given points of young pods with new spore suspension, and by measuring the inhibitory action of the diffusates on each day. It was shown that the production of PA decreased remarkably on the second day, and sank nearly to zero on the fourth day.
PA in the diffusate from the pods lost most of its inhibitory activity, when heated at 100°C for 5 minutes or diluted to 1:8.

Date of correction: February 19, 2009 Reason for correction: - Correction: CITATION Details: Wrong : 3) Müller, K. O.: Phytopath. Ztschr., 27: 237-254. (1954).

Right : 3) Müller, K.O.: Phytopath. Ztschr., 27: 237-254. (1954).

Date of correction: February 19, 2009 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Phytoalexin (PA for short) has been defined by Müller (1954) as antibiotics which are the result of an interaction between the host and the parasite, and which inhibit the growth of microorganism pathogenic to plants. In the present paper the results are dealt, of a study on the rate of production of PA, as affected by host plant conditions, and activities of PA, using soybean (Gylcine max Mer.) and Fusarium sp. which causes pod blight.
Drops of spore suspension of Fusarium were mounted on the inner surface of the seed rooms of the pods at different ages, and after centrifuging, the supernatant was tested for inhibitory action on the germination of fresh spores of Fusarium. It was found that the PA diffused distinctly into the drops placed on the young, unmatured pods, while hardly or not in the drops on the mature pods.
The PA productivity of the host tissue was determined by changing daily the drops placed on given points of young pods with new spore suspension, and by measuring the inhibitory action of the diffusates on each day. It was shown that the production of PA decreased remarkably on the second day, and sank nearly to zero on the fourth day.
PA in the diffusate from the pods lost most of its inhibitory activity, when heated at 100°C for 5 minutes or diluted to 1:8.

Corresponding author

Correction information

Register with J-STAGE for free!