On some properties of Phytoalexin produced as a result of the interaction between pea (Pisum sativum L.) and Ascochyta pisi Lib. I. On the activity as affected by ultraviolet irradiation and on some physico chemical properties of Phytoalexin.

Kazuo UEHARA

doi:10.3186/jjphytopath.23.230

On some properties of Phytoalexin produced as a result of the interaction between pea (Pisum sativum L.) and Ascochyta pisi Lib.

I. On the activity as affected by ultraviolet irradiation and on some physico chemical properties of Phytoalexin.

Kazuo UEHARA

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JOURNAL FREE ACCESS

1958 Volume 23 Issue 5 Pages 230-234

DOI https://doi.org/10.3186/jjphytopath.23.230

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Published: December 30, 1958 Received: - Available on J-STAGE: February 19, 2009 Accepted: October 27, 1958 Advance online publication: - Revised: -
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Date of correction: February 19, 2009 Reason for correction: - Correction: TITLE Details: Wrong : On some properties of Phytoalexin produced as a result of the interaction between pea (Pisum sativum L.) and Ascochyta pisi Lib
Right : On some properties of Phytoalexin produced as a result of the interaction between pea (Pisum sativum L.) and Ascochyta pisi Lib.

Date of correction: February 19, 2009 Reason for correction: - Correction: SUBTITLE Details: Wrong : I. On the activity as affected by ultraviolet irradiation and on some physicochemical properties of Phytoalexin
Right : I. On the activity as affected by ultraviolet irradiation and on some physico chemical properties of Phytoalexin.

Date of correction: February 19, 2009 Reason for correction: - Correction: ABSTRACT Details: Wrong : This paper reports some experiments on the nature of Phytoalexin (PA) produced as a result of the host-parasite interaction between pea, Pisum sativum L., and Ascochyta pisi Lib. Drops of spore suspension of A. pisi were placed on the seed rooms of pea pods and kept in moist chamber at 22°C. for 24 hours. These drops were then collected and centrifuged for 30 minutes at the rate of 3500r.p.m. The supernatant, PA-solution, was subjected to treatments shown below, and then placed on the slide glass in moist chamber with the addition of the spore suspension of A. pisi for the purpose of the germination test. The procedures were carried out under aseptic condition, as possible.
The experiments and the results were as follows:
1) PA-solution was irradiated by ultraviolet-ray for various minutes at a distance of 45cm from the energy source. The source of ultraviolet-ray was a Tôshiba lamp for sterilization (model 1510B, lamp GL-15×1). This lamp charged the rays between 1850 and 5780Å, but the greater parts were collected at 2537Å. The results showed that the activity of PA-solution was nearly lost by the exposure for 45∼60 minutes (Table 1 and Fig. 1).
2) When PA-solution with active carbon was added at a rate of 10gr. per liter and shaken for 5 minutes, the activity of the solution was lost (Table 2).
3) When PA-solution was dried in the air for 24 hours at 22°C., the inactivation of the solution was resulted (Table 3).
4) Both pH and specific electric conductivity of PA-solution were measured, using the glass electrodes pH-meter and Wheatstone bridge respectively. The pH value was 5.7∼5.8 and specific electric conductivity calculated from the electric resistance, was 164×10^-6mho. (Table 4).
Right : This paper reports some experiments on the nature of Phytoalexin (PA) produced as a result of the host-parasite interaction between pea, Pisum sativum L., and Ascochyta pisi Lib. Drops of spore suspension of A. pisi were placed on the seed rooms of pea pods and kept in moist chamber at 22°C. for 24 hours. These drops were then collected and centrifuged for 30 minutes at the rate of 3500 r.p.m. The supernatant, PA-solution, was subjected to treatments shown below, and then placed on the slide glass in moist chamber with the addition of the spore suspension of A. pisi for the purpose of the germination test. The procedures were carried out under aseptic condition, as possible.
The experiments and the results were as follows:
1) PA-solution was irradiated by ultraviolet-ray for various minutes at a distance of 45cm from the energy source. The source of ultraviolet-ray was a Tôshiba lamp for sterilization (model 1510 B, lamp GL-15×1). This lamp charged the rays between 1850 and 5780Å, but the greater parts were collected at 2537Å. The results showed that the activity of PA-solution was nearly lost by the exposure for 45-60 minutes (Table 1 and Fig. 1).
2) When PA-solution with active carbon was added at a rate of 10gr. per liter and shaken for 5 minutes, the activity of the solution was lost (Table 2).
3) When PA-solution was dried in the air for 24 hours at 22°C., the inactivation of the solution was resulted (Table 3).
4) Both pH and specific electric conductivity of PA-solution were measured, using the glass electrodes pH-meter and Wheatstone bridge respectively. The pH value was 5.7-5.8 and specific electric conductivity calculated from the electric resistance, was 164×10^-6 mho. (Table 4).

Date of correction: February 19, 2009 Reason for correction: - Correction: CITATION Details: Wrong : 1) Bovie, W. T.: Bot. Gaz., 61: 1-29. (1916).
3) Duggar, B. M. and A. Hollaender: J. Bact., 27: 219. (1934).
4) Fuchs, W. H. and Eva Kotte: Naturwissenschaften, 41: 169-170. (1954).
5) Gothoskar, S. S., R. P. Scheffer, M. A. Stahmann and J. C. Walker: Phytopath., 45: 303-307. (1954).
7) Haurowitz, F.: Chemistry and biology of proteins. (1950). p. 126-130.
9) Hollaender, A. and J. W. Oliphant: J. Bact., 48: 447. (1944).
10) Luria, S. E. and R. Latarjet: J. Bact., 53: 149. (1947).
11) McLaren, A. D. and S. Pearson: J. Polymer Sci., 4: 45-62. (1949).
12) Mitchell, J. S. and E. K. Rideal: Proc. Roy. Soc. London, Series A. 167: 342-367. (1938).
14) Müller, K. O.: Phytopath. Ztsch., 27: 237-254. (1954).

Right : 1) Bovie, W. T.: Bot. Gaz., 61: 1-29. (1916).
3) Duggar, B.M. and A. Hollaender: J. Bact., 27: 219. (1934).
4) Fuchs, W.H. and Eva Kotte: Naturwissenschaften, 41: 169-170. (1954).
5) Gothoskar, S.S., R.P. Scheffer, M.A. Stahmann and J.C. Walker: Phytopath., 45: 303-307. (1954).
7) Haurowitz, F.: Chemistry and biology of. proteins. (1950). P. 126-130.
9) Hollaender, A.and J.W. Oliphant: J. Bact., 48:447. (1944).
10) Luria, S.E. and R. Latarjet: J. Bact., 53: 149. (1947).
11) McLaren, A.D. and S. Pearson: J. Polymer Sci., 4:45-62. (1949).
12) Mitchell, J.S. and E.K. Rideal: Proc. Roy. Soc. London, Series A. 167: 342-367. (1938).
14) Müller, K.O.: Phytopath. Ztsch., 27: 237-254. (1954).

Date of correction: February 19, 2009 Reason for correction: - Correction: PDF FILE Details: -

Abstract

This paper reports some experiments on the nature of Phytoalexin (PA) produced as a result of the host-parasite interaction between pea, Pisum sativum L., and Ascochyta pisi Lib. Drops of spore suspension of A. pisi were placed on the seed rooms of pea pods and kept in moist chamber at 22°C. for 24 hours. These drops were then collected and centrifuged for 30 minutes at the rate of 3500 r.p.m. The supernatant, PA-solution, was subjected to treatments shown below, and then placed on the slide glass in moist chamber with the addition of the spore suspension of A. pisi for the purpose of the germination test. The procedures were carried out under aseptic condition, as possible.
The experiments and the results were as follows:
1) PA-solution was irradiated by ultraviolet-ray for various minutes at a distance of 45cm from the energy source. The source of ultraviolet-ray was a Tôshiba lamp for sterilization (model 1510 B, lamp GL-15×1). This lamp charged the rays between 1850 and 5780Å, but the greater parts were collected at 2537Å. The results showed that the activity of PA-solution was nearly lost by the exposure for 45-60 minutes (Table 1 and Fig. 1).
2) When PA-solution with active carbon was added at a rate of 10gr. per liter and shaken for 5 minutes, the activity of the solution was lost (Table 2).
3) When PA-solution was dried in the air for 24 hours at 22°C., the inactivation of the solution was resulted (Table 3).
4) Both pH and specific electric conductivity of PA-solution were measured, using the glass electrodes pH-meter and Wheatstone bridge respectively. The pH value was 5.7-5.8 and specific electric conductivity calculated from the electric resistance, was 164×10^-6 mho. (Table 4).

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