Volume 36 (1970) Issue 2 Pages 81-86
A virus was isolated from Narcissus poeticus L. showing yellow stripe using Gomphrena globosa by juice inoculation. The virus was readily transmitted by juice inoculation, but not by Myzus persicae. The inoculated leaves of G. globosa showed white necrotic local lesions 3-5 days after inoculation and became brownish within a few days. Systemic irregular lesions followed after about a week.
Of twenty plant species inoculated with the virus, fourteen were infected. Beta vulgaris, Vigna sinensis, Trifolium incarnatum, Nicotiana clevelandii, Sesamum indicum and Narcissus sp. were infected systemically without symptoms. Local necrotic or chlorotic lesions were produced on the inoculated leaves of Chenopodium amaranticolor, C. quinoa, Tetragonia expansa, Pisum sativum and Glycine max. Symptomless infection of the inoculated leaves occurred in Datura stramonium and Lycopersicon esculentum. The following species were not infected: Vicia faba, Cucumis sativus, Nicotiana glutinosa, N. tabacum (Bright Yellow), Allium fistulosum and Lilium formosanum.
The virus in vitro withstood heating at 60°C for 10 minutes, but not 65°C, dilution to 10-7 and 8 weeks of storage at 20°C, but not 16 weeks. In electron microscopy using dip method, long flexuous thread-like particles were found to be 450-600mμ in length, the mode being 500-550mμ.
Juice from inoculated G. globosa leaves reacted positively with the antiserum diluted to 1:128 in precipitation reaction. The virus, on the other hand, did not react with antisera to white clover mosaic virus, cymbidium mosaic virus, and potato virus X in precipitation reaction.
Owing to the similarity in host range, physical properties, particle morphology, and lack of aphid transmission, the virus was identified as narcissus mosaic virus (NMV) (Brunt, A.A.). NMV was detected from 34 of 41 specimens of narcissus showing yellow stripe, mosaic and necrotic flecks, and also from 5 of 12 plants showing no symptoms. Typical symptoms caused by this virus in narcissus are not determined, as yet, since virus-free seedlings for inoculation tests are not available at the moment.