Abstract
Endo type of pectin trans-eliminase (pectin lyase, PTE) produced by a pathogenic isolate of soft rot pseudomonad was distinctly separated into two fractions, PTE 1 and 11 by DEAE-cellulose chromatography. The PTE 1 was not absorbed with the ion exchanger, and purified 362-fold with a yield of 22.1% by means of NaCl gradient elution method on CM-cellulose column.
The purified enzyme was found to be homogeneous in free boundary electrophoretic and in ultracentrifugal analyses. The preparation showed the enzymatic activity of both tissue maceration and PTE, which were little different each other in several physical properties. It was indicated that the plant tissue maceration by a culture filtrate of this bacterium was caused by the endo-PTE.
