Cucumber mosaic virus (CMV) was purified by repeated precipitations with 8% PEG and 0.2M NaCl, followed by PEG solubility gradient (0∼6% PEG stabilized in 35∼5% sucrose) centrifugation. Virus yields ranged from 500 to 800mg per kilogram of fresh tissue. Purified preparation was highly infectious and contained uniform virus particles. Sedimentation pattern showing a single peak suggests that no aggregation occurred.
