Abstract
Pseudomonas eriobotryae phage (EP1) was precipitated from crude phage suspension by addition of 8% polyethylene glycol 6, 000 and 0.5M NaCl. The concentrated phage suspension was then purified by differential centrifugation and sucrose density gradient centrifugation. The antisera from rabbits immunized with the purified phage showed a titer of 1/2, 048 by a precipitin test.