Abstract
Two intracellular and an extracellular β-fructofranosidases (E.C. #3.2.1.26) (Int I, Int II and Ext I ) were obtained from the timothy leaf spot fungus, Cladosporium phlei and the culture filtrate, respectively, by DEAE Sephadex A-50 column chromato-graphy. All these enzymes hydrolysed sucrose, raffinose and β-2, 6-fructosan (prepared from the timothy haplocorm), but were incapable of hydrolyzing inulin and methyl-α-D-glucoside. Only Ext I showed higher affinity to β-2, 6-fructosan than to sucrose, differing from the two intracellular enzymes. The optimum pHs of Int I, Int II and Ext I were 5.0, 5.0 and 5.5, respectively, regardless of the substrates tested. Ext I was relatively heat stable as compared with Int I and Int II.
