Detection of Citrus Mosaic Virus by Enzyme-Linked Immunosorbent Assay

Abstract

Citrus mosaic virus (CiMV) was easily detected by enzyme-linked immunosorbent assay (ELISA) in various parts of satsuma orange plants, especially in newly sprouted leaves. Two to twenty ng/ml of CiMV and satsuma dwarf virus which is closely related serologically to CiMV could be detected by this technique. However, in the case of heterologous combination of coating γ-globulin and conjugate the ELISA values were reduced.