Abstract
Rocket immunoelectrophoresis (RIE) assay was applided for 9 plant viruses, barley stripe mosaic virus, tobacco mosaic virus, white clover mosaic virus, bean yellow mosaic virus, rice stripe virus, rice dwarf virus, cucumber mosaic virus, tobacco necrosis virus and its satellite virus. Agarose gels containing 4mM barbital, 20mM barbital sodium, 2mM EDTA, 200μg/ml sodium lauryl sulfate, 0.05-0.1% crude antiserum and 1% agarose, pH 8.6 were useful for virus assay. Barley stripe mosaic virus, tobacco mosaic virus and rice stripe virus were detected in 0.5μg/ml, 1μg/ml and 0.05 (in A260 value) of purified preparations, and in 1/256, 1/320 and 1/128 (W/V) crude saps from infected plants, respectively. Barley stripe mosaic virus was detected in about 250 protoplasts isolated from infected barley plants. The assay required only 5 to 7 hours for electrophoresis and staining. When two viruses were electrophoresed in the gel containing both antiserum, rocket-shaped precipitates from the same well were formed and each virus was assayed simultaneously.
