Abstract
Antibody activities of the antisera As-V-IM and As-Dp-IM which were obtained by injecting rabbits intramuscularly with cucumber mosaic virus and its D-protein, respectively, were associated with IgG class immunoglobulin. In addition to IgG class activity, the antiserum As-VF-IV which was obtained from the rabbit injected intravenously with formaldehyde-fixed virus had low levels of IgM class activity. Antibodies to the virions were isolated from the antisera by acid dissociation of virion-antibody immune complexes. Antibodies to D-protein were also purified by affinity chromatography on D-protein coupled Sepharose 4B. Cross-absorption tests with the unfractionated antisera and the isolated antibodies demonstrated that (1) antiserum As-VF-IV contained virion-specific and cross-reactive antibodies, (2) antiserum As-V-IM contained virion-specific, D-protein-specific and cross-reactive antibodies, and (3) antiserum As-Dp-IM contained D-protein-specific and cross-reactive antibodies.
IgG antibodies to virion and D-protein were also measured by F(ab')2-based enzyme-linked immunosorbent assay.
