All issues
Volume 51 (1985)
- Issue 5 Pages 521-
- Issue 4 Pages 399-
- Issue 3 Pages 249-
- Issue 2 Pages 127-
- Issue 1 Pages 1-
Volume 51, Issue 1
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
Lii Sin LEU, Hsiu Chu YANG1985 Volume 51 Issue 1 Pages 1-7
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe number of sclerotia of rice sheath blight fungus, Thanatephorus cucumeris (Rhizoctonia solani AG 1, Sasakii type), from single sampling spot (50×50×20cm), varied from 561 to 13 with an average of 156.5 after harvest of the first rice crop from 13 sampling locations in Taiwan in 1979. Sclerotia were most densely distributed on the surface layer (0-1cm) which contained 36.1 per kg dry soil. As the soil depth increased, the numbers of sclerotia decreased to 3.2, 3.3, 2.6, and 0.9 per kg dry soil, respectively, from 1-5, 5-10, 10-15 and 15-20cm depth. Per cent germination of the recovered sclerotia on 2 per cent water agar, in average, was 45.4, 19, 14.8, 8.5, and 5.5, respectively, from the surface to 20cm soil depth. The buoyant sclerotia in the paddy showed 14-40 per cent germination with average of 36.4 during 120 days growing period of the first rice crop in 1979. Sclerotia collected from the rice plants began to decrease in germination after being buried in soil for 4-6 months without regard to the cover crops. However, one year after burial, the sclerotia still showed 50-80 per cent germination. The germination of sclerotia kept at room temperature in the laboratory for 6 months declined to half and ceased 8-12 months later. The number of smaller sclerotia (those captured on 40 mesh screens after passing through 20 mesh screens) was 78.2% in average of those captured in the 20 mesh screen from the same sample while per cent germination from 40 mesh ones was almost half of those in the 20 mesh ones.View full abstractDownload PDF (931K) -
Takanori MAEDA, Narinobu INOUYE1985 Volume 51 Issue 1 Pages 8-15
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAntibody activities of the antisera As-V-IM and As-Dp-IM which were obtained by injecting rabbits intramuscularly with cucumber mosaic virus and its D-protein, respectively, were associated with IgG class immunoglobulin. In addition to IgG class activity, the antiserum As-VF-IV which was obtained from the rabbit injected intravenously with formaldehyde-fixed virus had low levels of IgM class activity. Antibodies to the virions were isolated from the antisera by acid dissociation of virion-antibody immune complexes. Antibodies to D-protein were also purified by affinity chromatography on D-protein coupled Sepharose 4B. Cross-absorption tests with the unfractionated antisera and the isolated antibodies demonstrated that (1) antiserum As-VF-IV contained virion-specific and cross-reactive antibodies, (2) antiserum As-V-IM contained virion-specific, D-protein-specific and cross-reactive antibodies, and (3) antiserum As-Dp-IM contained D-protein-specific and cross-reactive antibodies.
IgG antibodies to virion and D-protein were also measured by F(ab')2-based enzyme-linked immunosorbent assay.View full abstractDownload PDF (1611K) -
Yoshiaki CHIKUO, Toshiya SUGIMOTO1985 Volume 51 Issue 1 Pages 16-21
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA selective medium was developed for isolation of Aphanomyces cochlioides from the damped-off seedlings of sugar beet. The medium consisted of the following ingredients per litter of water: Corn meal agar (corn meal, 20g; agar, 20g); metalaxyl, 50mg; thiophanatemethyl, 50mg; chloramphenicol, 50mg and iprodione, 5mg. On this medium Aphanomyces cochlioides and A. euteiches exhibited a good hyphal growth and Pythium spp., Rhizoctonia solani, Fusarium spp., Alternaria sp., on the contrary, hardly grew or were impossible to grow. “C method” (microscopic observation after incubating the infected seedlings in sterilized water for 24 hours) was compared with the newly developed selective medium to detect A. cochlioides. Detection rate of A. cochlioides by means of both methods was about the same with each other. Recovery of A. cochlioides from the selective medium, however, was easy because of no development of associating organisms such as Pythium spp. and Rhizoctonia solani.View full abstractDownload PDF (870K) -
Masao GOTO, Hiroshi HYODO1985 Volume 51 Issue 1 Pages 22-31
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSStrains of Xanthomonas campestris pv. citri did not produce detectable amount of ethylene in yeast-extract peptone broth and a synthetic medium containing methionine. Citrus leaves inoculated with the bacterium, however, produced ethylene at three different stages, i.e., early (1 to 6h after inoculation), intermediate (10 to 24h after inoculation) and late (72 to 120h after inoculation until defoliation), respectively. Ethylene biosynthesis in the early stage was also demonstrated by the infiltration of extracellular polysaccharides (EPS) of the bacterium and various polysaccharides such as carboxymethylcellulose, xylan, polyethyleneglycol, star h, inulin, glycogen, pectin, and lipopolysaccharides extracted from the bacterium. The amount of ethylene produced with EPS was significantly higher than those with other polysaccharides as well as living cells of the bacterium. Citrus leaf tissues infiltrated with EPS showed no significant changes in the electrolyte leakage and amino acid composition. Ethylene production at the early stage of infection was considered to associate with the “early selective protection” of citrus leaf tissues. When the leaf water potential was increased by allowing detached leaves to absorb free water from the cut end of leaf petioles, water-congestion was reproduced even after 3 weeks on citrus leaves which had previously been injected with polysaccharides. Although the same phenomenon was observed with other polysaccharides, the degree of recovered water-soaking was most conspicuous with EPS. Bacterial cells suspended in water was effectively protected by EPS resulting in the marked increase in their survival rates.View full abstractDownload PDF (1326K) -
(III) Efficient Microinjection of Tobacco Mosaic Virus into Tomato Callus CellsHideyoshi TOYODA, Yoshinori MATSUDA, Tokuzo HIRAI1985 Volume 51 Issue 1 Pages 32-38
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe present study reports a procedure for efficient microinjection of tobacco mosaic virus (TMV) into tomato callus cells. The ‘plate-culture system’ was devised for injection and incubation of the cells. In the system, the cells were embedded in the solid medium and cytoplasmic streaming (CS) of the cells was directly observed with the aid of a phase contrast microscope of the injectoscope. The cells which showed the active CS were selected for injection. Success of injection was judged by a maintenance of CS in the cells after the glass pipette inserted was removed. Multiplication of TMV in the injected cells was detected by staining with fluorescein isothiocyanate-conjugated antibody. When the cells were stained soon after TMV injection, fluorescent cells were not detected. However, TMV-injected cells were stained when incubated for 2 days after injection. These results suggest that the present method is suitable for inoculating tomato callus cells with TMV and obtaining a high level of TMV multiplication in injected cells.View full abstractDownload PDF (2436K) -
Shinichi KUSAKARI, Yutaka TANAKA1985 Volume 51 Issue 1 Pages 39-42
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPythium ultimum Trow var. ultimum was isolated from soft-rotted fruits of strawberry in a plastic greenhouse of Osaka prefecture. Strawberry fruits infected by Pythium ultimum lost its color and changed like elastic rubber ball. These symptoms were distinguished clearly from the gray mold caused by Botrytis cinerea.View full abstractDownload PDF (1389K) -
Hideyoshi TOYODA, Hisako HASHIMOTO, Tokuzo HIRAI1985 Volume 51 Issue 1 Pages 43-46
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSInhibitory effect of culture filtrate (CCF) of Fusarium oxysporum f. sp. lycopersici on TMV infection was examined by using local lesion test. CCF, which was not dialysable against water, was inhibitive to TMV infection when applied before or after inoculation with TMV. CCF gave a positive reaction with tests for protein and carbohydrate. Both autoclaved and deproteinized CCF effectively inhibited TMV infection. The ethanol-precipitate obtained from deproteinized CCF was shown to be polysaccharides whose molecular weights were between 7×104 and 4×105 by gel permeation column chromatography.View full abstractDownload PDF (533K) -
1985 Volume 51 Issue 1 Pages 47-59
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2764K) -
1985 Volume 51 Issue 1 Pages 60-65
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1267K) -
1985 Volume 51 Issue 1 Pages 66-72
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1484K) -
1985 Volume 51 Issue 1 Pages 73-90
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (3829K) -
1985 Volume 51 Issue 1 Pages 91-104
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2905K) -
1985 Volume 51 Issue 1 Pages 105-112
Published: January 25, 1985
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1756K)
- |<
- <
- 1
- >
- >|