Abstract
The β-1, 3-glucan hydrolase activity increased in the leaves of Nicotiana glutinosa and N. tabacum var. Xanthi-nc inoculated with common strain of tobacco mosaic virus (TMV-OM) and in White Burley leaves inoculated with tomato strain of the same virus (TMV-T), but not in the leaves of White Burley and Xanthi inoculated with TMV-OM. In the experiment of artificial necrotization by treatment with hydrochloric acid and heated platinum ring, the increase of the activity was not recognized. On localization of the activity in the leaves of N. glutinosa inoculated with TMV-OM and of White Burley inoculated with TMV-T, the lesion areas showed higher activity than the marginal tissues around the lesions. When the inoculated detached leaf-halves of N. glutinosa were first incubated on distilled water at 25 and 35 C, then transferred to 25 C, a marked increase of the activity and formation of local lesions were recognized. However, when the same leaves were incubated continuously at 35 C, no increase of the activity and no local lesion were observed. Furthermore, in detached leaf-halves of N. glutinosa which were inoculated with TMV-OM and incubated on distilled water in dark and on 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) solution under light at 25 C, the dark- and DCMU-treatment induced the decrease of the activity and of formation of local lesions. However, such effect was not observed in the incubation on the glucose solution in dark. Using the polyacrylamide gel electrophoresis, the band of the enzyme from both TMV-OM-inoculated and noninoculated leaves of N. glutinosa, Xanthi and White Burley was single, but that from Xanthi-nc inoculated with TMV-OM and from White Burley inoculated with TMV-T was separated into two bands with a new additional one. In Nicotiana plants formed local lesion with inoculation of TMV strains, the enzyme activity was increased specifically. There was a change in the electrophoregram of the enzyme among the Nicotiana plant-TMV strain combinations.
