Abstract
Ferimzone (TF-164) did not significantly inhibit the syntheses of protein, DNA, RNA and cell walls in mycelia of P. oryzae. Ferimzone inhibited the uptake of 14C-acetate and 14C-pyruvate into cells and caused apparent incorporation inhibition of these precursors into lipids. Any individual components of lipids, however, were not found to be specifically inhibited by ferimzone in the incorporation of 14C-acetate and 3H-S-adenosylmethionine. Ferimzone did not inhibit the activity of fatty acid synthetase in vitro prepared from mycelia of P. oryzae. The incorporation of several amino acids including 3H-leucine into acid insoluble fraction was rather enhanced by ferimzone. In the present study any general metabolic pathways were not revealed to be significantly and specifically affected by ferimzone. In consideration of the fact that ferimzone caused specific leakage of acidic electrolytes from mycelia of P. oryzae, the results presented in this study suggested that ferimzone could disturb membrane permeability for specific molecules or ions in influx as well as in efflux.
