Increase in Cucumber Mosaic Virus Concentration in Japanese Radish Plants Co-infected with Turnip Mosaic Virus

(II) Electron Microscopic and Immunohistochemical Observations

Abstract

In Japanese radish plants infected with both cucumber mosaic virus (CMV) and turnip mosaic virus (TuMV), the concentration of CMV was significantly higher than in plants inoculated only with CMV. The distribution of viruses or viral antigens in inoculated plants was examined by electron microscopy and enzyme-immunohistochemistry. These observations indicated that the increase of CMV concentration was mainly due to an increase in the number of CMV-infected cells. Co-infection with TuMV had little effect on the cell-to-cell transport of CMV but significantly enhanced the long-distance transport of this virus.