Abstract
Stable hybridoma cell lines secreting monoclonal antibodies (MoAbs) to Fusarium oxysporum (860926a) were produced by fusing spleen cells from immunized BALB/c mice with SP2 myeloma cells. Antibody production by the cell lines, API9-2 and API9-3, was assayed by dot immunobinding assay (DIBA). These MoAbs showed positive reactions with 11 formae speciales (ff. sp.) of F. oxysporum, Gibberella fujikuroi (F. moniliforme), G. zeae (F. roseum), as well as with Nectria haematococca (F. solani). Whereas the MoAbs did not react with 35 plant pathogenic fungi, not classified in Fusarium spp. except N. solani (Thielaviopsis basicola). Thus these hybridoma-derived antibodies are considered to be specific to the genus Fusarium.
