Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Biological Activities of Toxin Produced by Tomato. Canker Bacterium, Clavibacter michiganensis subsp. michiganensis, against Tomato Plant and Its Callus Cells
Bernardo UENOTohru TERAOKADaijiro HOSOKAWAMinoru WATANABE
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1994 Volume 60 Issue 1 Pages 13-19

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Abstract

Clavibacter michiganensis subsp. michiganensis, causal agent of bacterial canker of tomato, produces phytotoxic glycopeptide which causes wilting on tomato cuttings. In order to select toxin-tolerant callus cells and to produce resistant tomato plants, the biological activities of a crude toxin against tomato plants and callus cells were compared with the pathogenic effects of the bacterium. In callus cells co-cultured with the bacterium, susceptible cultivars had a higher percentage of dead cells than resistant cultivars, which showed apparent differences in resistance. Similar responses were obtained with callus cells treated with a crude toxin precipitated with 30 to 40% saturation of ammonium sulfate. These results paralleled closely inoculation tests to whole plants. In tomato cuttings treated with unheated and heated (120°C, 5min) toxin transpiration rates decreased to 20 to 30% and wilting symptoms occurred both in the resistant and the susceptible cultivars, although the activity of heated toxin was attenuated. This result suggests that heat-labile component(s), such as protein(s), may be associated in the toxin activity, in addition to the heat-stable components, such as polysaccharides.

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