The composition of fruit tissue in the synthetic periclinal chimeras ‘NF-1’ and ‘NF-3’ that had been produced in order to try to give disease resistances into chimera plants were quantitatively analyzed with high performance liquid chromatography for four fiavanone glycosides. In the case of ‘NF-1’, juice sac (Germ layer I) showed a chromatogram very similar to that of ‘Kawano-natsudaidai’. Seed (Layer II), segment wall (Layer II and III), mesocarp (Layer II and III) and big vascular bundle (Layer III) showed chromatograms resembling those of ‘Fukuhara orange’. Similarly, in the case of ‘NF-3’, juice sac (Germ layer I) showed a chromatogram very similar to that of ‘Fukuhara orange’ (F), while the other tissues showed chromatograms very similar to those of ‘Kawano-natsudaidai’ (N). Consequently, the chimeral constitution in fruit of ‘NF-1’ is N-F-F for its first, second, and third germ layers, respectively, and F-N-N for ‘NF-3’. We propose the scientific names Citrus sinensis+natsudaidai for ‘NF-1’ and Citrus natsudaidai+sinensis for ‘NF-3’. We also propose the new variety name ‘FN-1’ instead of the earlier used ‘NF-1’. The proportions of four flavanone glycosides of mother varieties corresponded closely to the respective fruit tissues of ‘FN-1’ and ‘NF-3’. These results suggest that introducing tissue of a disease resistant variety to the second and third germ layers, and tissue of a high quality variety to the first layer will make a chimera tree with both disease resistance and high quality fruit.
