Cloning of a Region Encoding Multiple Polygalacturonases of Erwinia carotovora subsp. carotovora EC1

Abstract

A clone pEC301 which produces at least four polygalacturonases was obtained from a genomic library of Erwinia carotovora subsp. carotovora EC1 on plasmid pUC118. The results of restriction mapping of the 5.8kb EcoRI insert and of subcloning analysis indicated that a 1.8kb Ksp632I fragment produces at least four polygalacturonases. By isoelectric focusing followed by the activity staining for polygalacturonase, the pI of the four main Polygalacturonases were at 9.5, 6.0, 5.6 and 5.0. Subclones producing these polygalacturonases were able to macerate potato tuber tissue.