Abstract
A gene coding for toxoflavin biosynthesis-related protein-1 (TRP-1) of Pseudomonas (Burkholderia) glumae was cloned and sequenced. The gene coded a polypeptide of 244 amino acids with a calculated molecular mass of 27, 434 Da. Three motifs characteristic of methyltransferases were found in the deduced protein sequence of TRP-1, supporting the assumption that toxoflavin biosynthesis requires transmethylation. Mutant strains with a disrupted TRP-1 coding region by plasmid integration lost their ability to produce toxoflavin. The TRP-1 coding region was located in an operon structure consisting of several open reading frames. These results showed that the operon, including the TRP-1 coding region, was associated with toxoflavin production.
