2002 Volume 68 Issue 3 Pages 309-312
From lettuce (Lactuca sativa L.) with big vein symptoms, two bands were detected by Western blot analysis using antisera to Tobacco stunt virus and Tulip mild mottle mosaic virus (As-TMMMV), respectively. One band reacted to antiserum to Mirafiori lettuce virus (MiLV) stronger than As-TMMMV. Unstable filamentous Ophiovirus-like particles (LBV-O) decorated with As-TMMMV in immunogold labeling were observed along with Lettuce big-vein virus-like rod-shaped particles in diseased plants. Manual inoculation with lettuce sap caused chlorotic local lesions in Chenopodium quinoa and systemic infection in lettuce. Along with LBVV, LBV-O was transmitted by Olpidium brassicae. LBV-O was identified as MiLV for the first time in Japan.