The purpose of this study was to investigate the short-term biological effects of Excimer Laser. The surface of the skin in the rat was irradiated with KrF Excimer Laser at wave length of 248nm for 30 seconds at pulse repetition rate of 10Hz and pulse width of 15nsec.
Energy density was 3.1mJ/cm2, 47.6mJ/cm2, 160.0mJ/cm2, 576.9mJ/cm2 or 1714.2mJ/cm2.The irradiated skin tissue was removed of various times between 1 hour and seven days after rradiation and with H-E stain for pathological examination. Necrosis was observed in epidermis irradiated at 47.6mJ/cm2 and in dermis irradiated at 576.9mJ/cm2, and defect of the muscular tunic was observed in tissue irradiated at 1714. 2mJ/cm2. These results indicate that depth of skin defect is related to energy density and that necrosis occurs at energy density ranging from 3.1mJ/cm2 to 47.6mJ/cm2. Satisfactory wound was observed after irradiation at each energy density.
