Effect of Pulsing Electromagnetic Fields on the Proliferation and Differentiation of Osteoblast-like Cells

Studies on Using Immunohistochemical and Histochemical Staining Techniques

Abstract

An immediate obtainment of osseointegration would shortened the initial anchorage period of implant within bone and setting time of the final superstructure. Application of pulsing electromagnetic fields (PEMFs) as a method for increasing the rate of osseointegration has been considered. PEMFs have been demonstrated to promote the proliferation and differentiation of osteoblast-like clone MC3T3-E1 cells.
Recently, the immunohistochemical staining technique having Brd U or PCNA has been used as markers to study the proliferation dynamics of S-phase cells or late G, to S-phase cells. In this study, cell proliferation was examined in the experimental group having PEMFs-stimulation and in the control group lacking stimulation by immunohistochemical staining technique using Brd U and PCNA monoclonal antibodies. Brd Ulabeling and PCNA- positive indices were respectively calculated and compared. Further, alkaline phosphatase activity was investigated as a marker for cell differentiation of MC3T3-E1 cells by using a histochemical staining technique.
The results suggested that the Brd U-labeling index and PCNA-labeling index distinctly increased in the experimental group (P<0.001). Cells in the S-phase and late G, to S-phases were 1.8 times and 1.6 times higher, respectively, than control. Alkaline phosphatase staining was stronger in the experimental group than in the control group.