デンチャープラークおよび歯石様沈着物の電子顕微鏡的観察

抄録

The formation processes of the denture plaque and calculus were studied by means of transmission electron microscopy and EPMA. The dentures worn experimentally for 6 hours and 3 days and the dentures obtained from patients were used.
For the electron microscopy, the pieces cut from the dentures were embedded in Epok 812 after fixation, and the ultrathin sections were examined without staining. For EPMA, the cut pieces were immediately frozen in liquid nitrogen, and the freeze-dried sections were prepared and examined.
Amorphous electron dense materials appeared in the spaces between the loosely bound microorganisms on the denture surface at 3 days, although the denture plaque was not observed at 6 hours. The relatively high X-ray peak of calcium was detected from the plaque. The lamellated substance appeared on the surface of the denture from the patients, and the substance was heavily mineralized. The initial mineral crystals appeared along the plasma membrane of microorganisms, the intermicrobiol matrix, and the lamellated substance.
These results suggested that the denture plaque facilitates the calcium accumulation and the formation of the denture calculus is initiated by the intermicrobiol matrix, the cell membrane, and/or the lamellated substance.