Abstract
Purpose: The purpose of this study was to evaluate biological effects (i. e. cytotoxicity and interleukin 1β production) of eluates from commercially available denture adhesives (7 cushion-types, 5 creamtypes, 1 powder-type, and 2 seal-types) on human gingival fibroblasts.
Materials and Methods: To achieve this purpose, defined amounts of commercially available denture adhesives were dialyzed (cut-off MW: 12, 000 to 14, 000) against Dulbecco's Modified Eagle Medium for one or two days. The cytotoxicity and interleukin 1β inductions of these eluates were analyzed by aqueous soluble tetrazolium/formazan assay (MTS assay) and enzyme-linked immunosorbent assay (ELISA), respectively.
Results: The cytotoxicity against human fibroblast cells of the materials was varied, to a lesser or greater extent, depending on the type of materials. Particularly, cream-type denture adhesives caused much population of cell death, whereas cushion-type materials showed neither cytotoxicity nor induction of interleukin 1β. Though several materials acidified the dialysates, such a pH change was suggested to have no effects on cell viability.
Conclusion: The results of this study implied that several commercially availfable products of denture adhesives may be cytotoxic to human oral tissues in vivo.
