キンギョの脳内リボ核酸合成と受動的回避潜時に及ぼすアクチノマイシンDの効果

大井 修三

doi:10.4992/jjpsy.46.191

THE EFFECTS OF ACTINOMYCIN D ON THE BRAIN RNA SYNTHESIS AND ON THE PASSIVE AVOIDANCE LATENCY IN THE GOLDFISH

SHUZO OHI

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JOURNAL FREE ACCESS

1975 Volume 46 Issue 4 Pages 191-198

DOI https://doi.org/10.4992/jjpsy.46.191

[Correction] The Japanese Journal of Psychology Vol.46 No.5 (1975) pp.304-304

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Published: November 30, 1975 Received: November 12, 1974 Available on J-STAGE: July 16, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: July 16, 2010 Reason for correction: - Correction: ABSTRACT Details: Wrong : Two experiments were conducted which were designed to examine the biochemical hypothesis of the memory consolidation with actinomycin D (Act-D), a strong RNA synthesis inhibitor.
In Exp. I, the effect of Act-D on the brain RNA synthesis and its time course was examined in the goldfish by means of incorporation of ³H-uridine into the brain RNA. Act-D (2μg/10μl/fish) was injected intracranially at 0.5 (n=4), 3 (4), 24 (3), 48 (4), and 72 (4) hours prior to intracranial injection of ³H-uridine (5μCi/5μl/fish), 0.5 hour before the S was sacrificed. The control group (3) (shown as 0 in Fig. 1 and Table 1) was given the same treatment without Act-D injection. At 0.5hour, about 60% of the RNA synthesis was inhibited. At 3 hours, about 70% of it (maximum effect) was found inhibited and this continued even 4 days afterwards (see Fig. 1 and Table 1).
In Exp. II, the effect of Act-D on the passive avoidance latency was examined in the goldfish. The Act-D group (n=16) was injected Act-D (2μg/10μl/fish) while the control group (n=16) the distilled water (10μl/fish). The injection was intracranial and more than 3 hours before the first day training. The Ss were given the passive avoidance training for 4 days (12trials/day). The apparatus was shown in Fig. 2. The Ss were given the unconditioned stimulus, 4V a. c., immediately after they went out of a starting box. Then the latency (the time elapsed till the S went out: the maximum latency=120sec) was measured. The difference between the groups and the group×day and the group×day×block interactions were either significant or approached the level of significance (p<.05, p<.10, p<.05, respectively), but the group×block interaction was not significant (see Fig. 3). These results suggest that under the condition of 70% inhibition of the brain RNA synthesis, intraday-acquisition was established on each day but interday-retention was markedly blocked. As the former mainly corresponds to the short-term memory and the latter to the long-term memory, the present results might be taken to the hypothesis that the brain RNA synthesis is necessary to the memory consolidation.
Right : (a) Intracranial injection of actinomycin D (2μg/10μl/fish) inhibited about 70% of the goldfish brain RNA synthesis from 3 hours to 4 days later. (b) The goldfish injected actinomycin D intracranially was given the passive avoidance training. Intraday-acquisition (short-term memory) was not blocked but interday-retention (long-term memory) was markedly blocked. It might suggest that the brain RNA synthesis is not related to short-term memory but is necessary for long-term memory.

Abstract

(a) Intracranial injection of actinomycin D (2μg/10μl/fish) inhibited about 70% of the goldfish brain RNA synthesis from 3 hours to 4 days later. (b) The goldfish injected actinomycin D intracranially was given the passive avoidance training. Intraday-acquisition (short-term memory) was not blocked but interday-retention (long-term memory) was markedly blocked. It might suggest that the brain RNA synthesis is not related to short-term memory but is necessary for long-term memory.

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