Analysis of Urinary Modified Nucleoside in Lung Cancer Cases

Abstract

Urinary concentration of pseudouridine, primarily a degradation product of transfer ribonucleic acid, was determined in 35 patients with lung cancer and 24 controls by high-performance liquid chromatography. Urinary pseudouridine concentrations of patients with lung cancer were significantly higher than those of controls, they were 37.3±15.7nmol/μmol creatinine and 23.4±5.1nmol/μmol, creatinine respectively (p<0.001). No significant difference was observed in urinary pseudouridine concentration with respect to each cell type. At two cut-off levels, 30nmol/μmol creatinine and 25nmol/μmol, creatinine sensitivity was 65.7% and 88.6%, and specificity was 83.3% and 66.7%, respectively. Sensitivity increased to 97.1% at a cut off level of 25nmol/μmol creatinine with combined use of serum CEA value. These results suggest that urinary pseudouridine concentration is a useful marker in patients with lung cancer.