A study on specimen treatment using rehydration method

Abstract

Objectives : For samples expected to be dried during preparation, a method to prepare samples with a high diagnostic value by employing rehydration was investigated.
Study Design : Impression preparations of tissues histologically diagnosed as adenocarcinoma were prepared. After drying, the preparations were rehydrated with saline or 10% skim milk, and the nuclear findings and area were investigated.
Results : The nuclei in cells after rehydration were slightly larger than those in the standard alcohol-fixed preparation, but close observation of the nuclei and cytoplasm was possible.
Conclusion : For samples likely to be dried during preparation, preparations with a high diagnostic value can be prepared by rehydration with saline after preparing dry samples.