Diagnostic accuracy of BD SurePath^TM liquid based cytology in endoscopic retrograde cholangiopancreatography

—A Direct-to-vial study—

Abstract

Objective : We assessed the diagnostic accuracy and specimen adequacy of endoscopic retrograde cholangiopancreatography (ERCP) brush cytologies prepared with the BD SurePath^TM method.
Study Design : We examined 31 cases with ERCP brush cytology from April 2013 to December 2014. We cut the brush head, rinsed it out in a vial with the CytoRich Red^TM fixative and prepared a specimen with the BD SurePath^TM method. These cases were classified as negative, suspicious, positive, and unsatisfactory and compared with the histological diagnosis.
Result : Of the 31, 9 were negative, 8 suspicious, 14 positive and 0 unsatisfactory. Of the 14 positive, 7 were diagnosed as an adenocarcinoma from the histology. Cells in a defined 13-mm diameter area were distributed in thin layers. Air-drying artifacts and crush artifacts were removed from all specimens, and good preservation was ensured.
Conclusion : The BD SurePath^TM method enabled the preparation of good specimens in which many cells were collected and from which unsatisfactory factor could be removed, thereby enhancing the diagnostic accuracy of ERCP brush cytology.