2016 Volume 55 Issue 4 Pages 231-238
Objective : Tumor cells of Grade 1-2 follicular lymphoma (FL G1-2) can be difficult to distinguish from non-neoplastic cells in cytology specimens. Therefore, we developed double immunostaining for BCL2 and BCL6 as a tool to differentiate FL G1-2 cells.
Study Design : Cytology specimens from 50 cases, including 35 cases of malignant lymphoma and 15 cases of non-neoplastic lesions, were used for this study. All samples were collected from patients who had undergone surgery and received a final pathological diagnosis at our hospital during the 16-year period from 2000 to 2016. Double immunostaining was performed on the resected specimens.
Results : All eight cases of FL G1-2 and 22 out of the 29 cases of malignant B-cell lymphoma were doubly stained for both BCL2 and BCL6, and were designated as positive. In contrast, all 15 cases with non-neoplastic lesions were negative. Malignant T-cell lymphoma and classical Hodgkin’s lymphoma were negative.
Conclusion : Double immunostaining for BCL2 and BCL6 could be a powerful auxiliary tool for the diagnosis of FL G1-2.
