Abstract
Objective: We evaluated the applicability and usefulness of immunocytochemical staining for cyclin A, p53, ER-a, PR-B in preoperatively predicting the prognosis of endometrial carcinoma using endometrial cytology with the Thinlayer method.
Study Design: Cytological specimens from 44 patients with endometrial carcinoma prepared with the Thinlayer method were used. The results of immunocytochemical staining for cyclin A, p53, ER-a, and PR-B were compared to clinicopathological parameters.
Results: Patients positive for cyclin A and p53 and negative for PR-B and ER-a appeared to have unfavorable clinicopathological characteristics such as high grade histology, advanced clinical stage, lymphovascular space involvement (LVSI), and deeper myometrial invasion (MI). In contrast, patients positive for ER-a and PR-B and negative for cyclin A and p53 had favorable characteristics such as well-differentiated tumor, early clinical stage, negative LVSI, and less MI. Immunocytochemical results between preoperative and operative specimens correlated positively.
Conclusion: Consistent specimens prepared using the Thinlayer method enabled multiple immunocytochemical staining. Endometrial cytology using the Thinlayer method is useful in predicting the prognosis of endometrial cancer before therapy.
