Abstract
A new ascites-type cell, NAs-1, was obtained in culture from mouse neuroblastoma C1300. The cells were growing anchorage independently in F-10 medium containing 10% FCS, and retained the ability of growing and producing ascites fluid when intraperitonealy injected into mice. A morphology of NAs-1 growing in culture medium was small and round without any neuronal processes. Karyotype analysis showed a homogeneous chromosome number, 40, with a marker chromosome [t(13: 16)] and a minichromosome. Catecholamines, norepinephrine and dopamine, were found in the cell extracts and the contents of dopamine was particularly high. Neuron specific enolase (γ-subunit) was also detected. The treatment of cells by dibutyryl cyclic AMP, prostaglandin E_1, or BL191 (phosphodiesterase inhibitor) induced the biochemical differentiation in terms of catecholamine and cyclic AMP contents, but failed to promote typical morphological differentiation including the extention of process or the significant pro-motion of adherence onto the flask surface. 15 day after intraperitoneal injection, most of NAs-1 invaded along peritoneum and brougt about infiltrative metastasis in varied organs such as liver, kidney, lung, and esophagus.
