2000 Volume 36 Issue 7 Pages 1034-1041
Purpose : The purpose of this study was to determine the effect of total parenteral nutrition (TPN) and erental nutrition (EN) on the septic status of the rat by measuring the serum endotoxin, β-_D-glucane and cytokines and analyzing histological changes of the liver and the lung. Methods : Sprague-Dawley male rats, each weighing about 200 g, were randomly divided into four groups. Group I (control group) was fed a rat chow diet for 5 days. In group II (PO+LPS group), rats were fed a rat chow diet for 5 days, then received an intraperitoneal injection of endotoxin (Escherichia coli LPS) 2 mg/kg body weight. In group III (TPN+LPS group), rats received a solution of 20% glucose, 3.3% amino acids and 0.5% fat intravenously as TPN for 5 days followed by intraperitoneal LPS injection. In group IV (EN+LPS group), rats received same TPN solution through the gastrostomy for 5 days followed by intraperitoneal LPS injection. Rats were killed 3 hours after injections of LPS. We measured serum levels of endotoxin, β-_D-glucane and cytokines (TNF-α, IL-1β, IL-6, IL-8, IFN-γ). Pathological change in the liver and the lung were evaluated with HE-staining and immunohistochemical staining. Results : In septic groups (group II, III and IV), serum endotoxin levels were increased. However, β-_D-glucane level was increased only in group III. The serum cytokines levels were increased in all septic groups. In group III, the liver showed remarkable dilatation of the sinusoid, ballooning of the hepatocyte and bleeding. The lung showed edema and bleeding. Conclusions : These results suggest that the TPN+LPS group might develop multiple organ failure (MOF) in septic status. On the other hand, the EN+LPS group showed minimal change in the liver and the lung, which suggested that EN might suppress the development of MOF in the septic status.
