Abstract
We developed an anti-thrombotic catheter, whose surface was coated with both urokinase and heparin, and evaluated its anti-thrombotic potential. A modified Chandler loop test revealed that the catheter kept high anti-thrombotic capacity even after 25 days'rinsing with human citrated whole blood. Activities of urokinase and heparin measured by synthetic substrate for plasmin and thrombin respectively were reserved even after 25 days'rinsing. Both TAT and D-dimer in blood in the modified Chandler loop increased gradually in a time dependent manner, which suggests that anti-thrombotic function of the catheter were reserved for the evaluated period by two distinct mechanisms of anticoagulation by heparin and of fibrinolysis by urokinase. These ex-vivo studies confirmed the long-lasting anti-thrombotic efficacy of anti-thrombotic catheter coated with both urokinase and heparin, and suggest promising usefulness of its clinical application.
