二次性ビタミンK欠乏症の1例と生後1か月の幼若乳児37例におけるプロトロンビンの二次元免疫電泳での検討

抄録

Two-dimensional immunoelectrophoresis of prothrombin was carried out on plasma samples of 6 month old patient with secondary vitamin K deficiency and thirty seven young infants aged one month.
In the patient with secondary vitamin K deficiency, abnormal prothrombin, which showed faster mobility in the presence of calcium ions, already appeared at 72 hours before onset of G. I. bleeding and coagulation disorder due to vitamin K deficiency, although normal prothrombin level was within the normal limits (Fig. 1, 2).
At 48 hours before onset, abnormal prothrombin increased to the same level as normal prothrombin which had decreased (Fig. 2).
Thereafter, coagulation disorder due to vitamin K deficiency appeared, because of lower level of normal prothrombin.
Thus, we could detect the mild vitamin K deficient state by two-dimensional immunoelectrophoresis of prothrombin, before the actual bleeding episode or prolongation of prothrombin time, Thrombotest and Hepaplastintest took place.
We found that about one-third of young infants aged one month had various level of abnormal prothrombin regardless of variety of feeding method, although normal prothrombin level was within the normal limits (Fig. 3). Based on these data, we concluded that about one-third of young infants aged one month were in mild vitamin K deficient state.
Those mild vitamin K deficient state could not be detected by the ratio of procoagulant activity to antigen of prothrombin, Thrombotest and Hepaplastintest (Fig. 4, 5).