Abstract
When plasmin was mixed with α2M or α2PI, plasmin was inhibited faster by α2PI than by α2M. When plasma was mixed with UK and fluorescamine-labeled casein (f-casein), hydrolysis of f-casein was observed 10min after incubation started, and increased significantly after 20min incubation. Crossed-immunoelectrophoresis indicates the presence of free α2PI and α2PI-plasmin complex at 10, and 30min incubation. These results indicate that plasmin, α2PI and α2PI-plasmin complex could coexist in the plasma in contrast to a purified system.
