抄録
Although studies of UK have been developed, it is still uncertain as to the presence of UK in the circulating blood. In addition, the relation between UK antigen and its inhibitor was not fully examined. The present study was carried out to elucidate these problems.
Anti-UK serum used in this experiment was obtained from rabbit, immunized by low molecular weight UK and showed a single arc on Ouchterlony's immunodiffusion or immunoelectrophoresis. This anti-serum neutralized UK activity. The relationships between UK antigen, UK activity and α2PI antigen in the healthy adults or patients with thrombosis were examined. The correlation between UK antigen and UK activity was r=0.36 (p<0.05), UK activity and α2PI antigen was r=-0.1 (p<0.05), and UK antigen and α2PI antigen was r=0.23 (p>0.05).
In the experiment of gel filtration of UK and plasma on Sepharose 2B, UK activity was decreased and UK antigen was migrated more anode region on Laurell electrophoresis than UK only, when UK was incubated with plasma.
Normal plasma or plasma with thrombosis were eluted on anti-UK Sepharose column chromatography, electrophoresed on SDS polyacrylamide. Several protein bands were migrated on polyacrylamide gel. These results suggest the possibility that UK is present in the circulating blood, forming a complex with inhibitor. However, further investigation is required to resolve this possibility.
