抄録
The immunological properties of Bilokinase (BK) were elucidated by comparing them to those of Urokinase (UK). Highly purified BK and UK were prepared from bovine bile and bovine urine, respectively, and both activators were administered to rats or rabbits with Freund's complete adjuvant for 2 months. The antisera obtained showed a specific reaction in the precipitin formation against their own antigen, but did not show a cross reaction to the other antigen. It was also demonstrated that the BK activity was suppressed only by the anti-BK antiserum and not by the anti-UK antiserum which inhibited UK activity.
In addition, anti-human UK antiserum which was prepared by immunizing rabbits with commercial human UK, further purified in our laboratory showed a strong inhibitory effect on the activity of human UK, whereas the activity of human BK which was extracted from the bile of patients with cholelithiasis was not inhibited by the anti-human UK antiserum.
These results strongly suggest that these two activators are antigenically distinct proteins.
