デキストラン硫酸およびヘパリンの抗凝固作用
主としてフィブリン体への影響
1982 年 13 巻 4 号 p. 585-588
詳細
1982 年 13 巻 4 号 p. 585-588
The inhibitory effects of respective DS and heparin on the fibrinogen-fibrin conversion were studied with purified bovine fibrinogen and thrombin as reagents.
1) Both DS (0.05-0.8mg/ml) and heparin (1.0-5.0u/ml) showed inhibitory effects on the whole fibrin polymerization. Further study was done at every step of all the reactions of fibrinogen-fibrin conversion.
2) The fibrinopeptide release which reflects the proteolysis of fibrinogen induced by thrombin was measured. Heparin inhibited the reaction, but DS didn't at this step.
3) Then both accelerated the fibrin monomer polymerization which reflects the reaction from fibrin monomer to fibrin.
These results showed that the fibrinogen-fibrin conversion was eventually inhibited by either DS or heparin, independent of AT III. The inhibition by heparin operated mainly at the step of thrombin-fibrinogen reaction, though fibrin monomer polymerization seemed to be accelerated later. While the inhibitory mechanisms by DS could not be clarified. These results also suggest that in coagulation studies with heparin or DS administered, there is a possible discrepancy between the anti-thrombin activities estimated by clotting time and those by chromogenic substrate.
