PGI₂ and TXA₂ syntheses from arachidonic acid in isolated glomeruli

Abstract

PGI₂ and TXA₂ synthesis in glomeruli isolated from rat kidney was investigated and following results were obtained. PGI₂ and TXA₂ were measured by RIA kit (New England Nuclear). TXB₂ production was increased by addition of arachidonic acid, but synthesis of 6-keto-PGF_1α was slightly increased. The recovery of added TXB₂ in presence of indomethacin was almost 100%. On the contrary, the recovery of added 6-keto-PGF₁ was remarkably reduced. From these results it was concluded that 6-keto-PGF_1α might be farther metabolized in glomeruli. TXA₂ synthesis was inhibited by addition with aspirin (ASA), Indomethacin and OKY 1581. Dipyridamole slightly inhibited TXA₂ production in vitro and in vivo, and increased PGI₂ production in vivo, while Trapidil inhibited TXA₂ production in vivo and more remarkably increased PGI₂ production in vivo.