血液と脈管
Online ISSN : 1884-2372
Print ISSN : 0386-9717
血漿中のGlu-プラスミノゲン, Lys-プラスミノゲンの高性能アフィニティクロマトグラフィーによる直接的分析
伊藤 尚史野口 康二風間 睦美笠井 献一
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1986 年 17 巻 3 号 p. 282-285

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Glu-plasminogen (Glu-Plg) and Lys-Plg were specifically separated with high-performance affinity chromatography, but it was almost impossible to analyze them in human plasma in a short time because of the existence of a large amount of other components. This problem was solved by introducing specific detection system. Plasminogens were specifically detected by plasmin activity which was produced by the on-line activation using urokinase. A small amount of human plasma (40μl) without pretreatment could be directly applied to the column packed with an adsorbent coupled with p-aminobenzamidine (Asahipak GS520-AHA-ABA; Asahi Chemical Industry Co.). The existence of Lys-Plg was observed in most plasmas. When urokinase was added to the normal plasma, Lys-Plg disappeared faster than Glu-Plg and active plasmin was not detected at all. These findings directly indicate that Lys-Plg is more susceptible to activation by urokinase than Glu-Plg and that the active plasmin in plasma is immediately trapped by inhibitors. Differences between normal and hereditary abnormal plasma was also clearly observed. This new system should be widely applicable in many fields, e. g., in clinical diagnosis and a therapeutic monitor for thrombosis.

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