PAF-Acether Induced Phenomena in Guinea-Pig Arteries
Ultrastructual and Optoelectronic Observations
1988 年 19 巻 6 号 p. 566-577
詳細
1988 年 19 巻 6 号 p. 566-577
In this study electron microscopic and optoelectronic observations on arterial thrombi induced in the guinea pig by inflammatory inducing maneuvers such as electrical challenge, topical superfusion by PAF-acether or the combination of both are described. These maneuvers result within minutes in a well defined sequence of phenomena: the endothelial cells retract and develop blebs, platelets adhere onto the exposed intimal tissue, aggregate, degranulate and conflate into a thrombus. Recruitment of leukocytes occurs within 10 minutes and blood coagulation is triggered later on. Phagocytosis of platelets and fibrin by the invading cells is a constant finding but is insufficient to offset the propensity to vascular occlusion. The early platelet adhesion and aggregation as well as the recruitment of leukocytes is inhibited by specific PAF-acether antagonists, such as BN 52021 and 48740 RP. These observations indicate that arterial thrombosis induced by these techniques shares features pertaining to the inflammatory process.
