Abstract
Platelet transfusion to neonates is usually not performed directly from a transfusion bag, but by syringe. We examined the effectiveness and safety of platelet transfusion by syringe.
Changes in oxygen and carbon dioxide concentration, lactate, pH, glucose, and platelet aggregation were examined for four types of apheresis platelet preparation: agitated at room temperature (apheresis bag), transferred to a separation bag and agitated at room temperature (separation bag), drawn into a syringe and agitated at room temperature with the addition of air (air-added syringe), and drawn into a syringe and allowed to stand at room temperature with air removed (air-removed syringe).
No changes were observed between the apheresis bag and separation bag even after 6 hours. Oxygen concentration in the air-removed syringe was significantly decreased after 2 hours. Carbon dioxide concentration in the air-removed syringe was significantly increased after 4 hours, lactate was increased, and pH, glucose and platelet aggregation were decreased. With regard to the air-added syringe, changes could be prevented by agitation.
For neonatal transfusion, a separation bag is considered to be an effective and safe method. Transfer of small amounts to a bag for transfusion is therefore desirable.
This study reports on in vitro effects only, and in vivo effects should be reviewed with respect to post-transfusion survival and recovery rates in order to make clinical decisions.
