CD34-POSITIVE CELL COLLECTION EFFICIENCY IN PERIPHERAL BLOOD STEM CELL HARVEST USING A SINGLE DOUBLE-LUMEN CENTRAL VENOUS CATHETER

Abstract

Peripheral blood stem cells (PBSCs) are generally collected using two peripheral venous catheters (PVCs): one for drawing blood and the other for returning processed blood. A single double-lumen central venous catheter (dCVC) can alternatively be used for PBSC harvest (PBSCH). It is occasionally feared that the processed blood might dilute the stem cell concentration in blood drawn using dCVC, decreasing CD34-positive cell collection efficiency. However, there are no obvious data suggesting that collection efficiency using dCVC is inferior to that using PVC. In this study, we defined [the number of collected CD34-positive cells (counts) ]/[the number of CD34-positive cells in peripheral blood (counts/ml) × processed blood volume (ml) ] as estimated collection efficiency and retrospectively evaluated the collection efficiency of 64 PBSCHs from 36 patients with hematological malignancies. The estimated collection efficiency using dCVC was not inferior to that using PVC. Therefore, PBSCH using dCVC is considered not to be inferior to that using PVC in terms of CD34-positive cell collection efficiency.