PERCENT RECOVERY OF FIBRINOGEN IN CRYOPRECIPITATE IS AFFECTED BY FIBRINOGEN CONTENT IN FRESH FROZEN PLASMA

Abstract

The "Protocol for in-house production of cryoprecipitate" was published by the Japan Society of Transfusion Medicine and Cell Therapy in 2016. It outlines the standard operating procedures for the preparation of cryoprecipitate (CRYO), and recommends measuring the percent recovery of fibrinogen in CRYO at least once every six months for quality control. Based on the protocol, we evaluated 51 CRYO preparations produced in our hospital, derived from 51 fresh frozen plasma (FFP) preparations distributed by Japan Red Cross Blood Center.

The mean±standard deviation (SD) volume of the CRYO preparations was 56.1±6.4 ml. There was a significant correlation between the fibrinogen content of CRYO and FFP preparations (r = 0.965; p < 0.001). The mean±SD fibrinogen content of CRYO preparations was 528.3±152.1 mg, and the maximum difference among CRYO preparations was 655.2 mg. The mean±SD, median, maximum and minimum percent recovery of fibrinogen in CRYO preparations was 48.0±6.2%, 48.2%, 60.5% and 33.1%, respectively. There was a significant correlation between the percent recovery of fibrinogen in CRYO and the fibrinogen content in FFP preparations (r = 0.788; p < 0.001). This suggests that we should consider not only problems with preparation techniques but also low fibrinogen content of FFP preparations when percent recovery of fibrinogen in CRYO is below 40%.