ひらたけ (pleurotus ostreatus) および かんたけ (pleurotus spodoleus) レクチンの特異性について

抄録

Pleurotus ostreatus and Pleurotus spodoleucus extracts, after heating at 56°C for 30 minutes, agglutinate O red cells most strongly and AB red cells most weakly. The activity of Pleurotus extract, present in the fraction precipitated at 0.3 and 0.6 saturation with (NH₄)₂ SO₄, is inhibited by O secretor saliva and Shigella dyselteriae polysaccharide, but not by O non-secretor saliva and Type XIV Pneumococcus polysaccharide.
As the result of hemagglutination inhibition test with sugars, these Pleurotus lectins are inhibited by 2′-fucosyllactose most strongly, D-galactose and lactose more strongly than melibiose, D-galactosamine and L-rhamnose, but not inhibited by L-fucose, D-glucose, D-mannose and D-glucosamine.
Treatment of group O red cells and O secretor saliva with H-decomposing enzyme (α-L-fucosidase) from Bacillus fulminans resulted in the complete loss of reactivity with both anti-H eel serum and pleurotus ostreatus lectin.
Pleurotus ostreatus and Pleurotus spodoleucus lectins react specificially with H substance (O substance and Eisler-Kagaya's heterophile anti-gen) and are assumed to be analogous to anti-Shigella dysenteriae immune chicken serum and Laburnum alpinum lectin.