CONVERSION OF GROUP O RED CELLS BY TREATMENT WITH SERUM FROM GROUP Bm OR Cis AB SUBJECTS AS THE ENZYME SOURCE

Abstract

When group O red cells were incubated with UDP-Galactose and serum from group B or AB subjects, they became strongly agglutinable by human anti-B serum. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:128.
Group O red cells incubated with UDP-Galactose and serum from group B_m or A₁B_m subjects were converted to B active cells. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:8 or 1:16.
Comparison of the agglutination titers of the converted cells against human anti-B serum shows that the activity of serum α-galactosyltransferase from group B_m subjects is approximately one-eighth or one-sixteenth of that from group B subjects.
When group O red cells were incubated with UDP-Galactose and serum from group Cis AB subjects, they could not be agglutinated by human anti-B serum. The activity of serum α-galactosyltransferase from group Cis AB subjects could not be demonstrable by agglutination test. This is considered to be associated with the regularly present cold anti-B agglutinins in the sera of group Cis AB subjects.