In this study, we evaluated the peripheral blood stem cell collection performance of a new fully automatic type cell separator Baxter Amicus, in comparison with a manual program, COBE Spectra. Patients scheduled to receive autologous peripheral blood stem cell transplantation (PBSCT) were alternately distributed to the Amicus and Spectra groups, and the collection efficiency of CD34-positive cells and the performance of the collection products were compared between the two groups.
In the first study, Amicus was used with the default settings (MNC offset: 2.3m
l, processing blood volume: 1, 400m
l×7 cycles). The number of patients was five in both groups, with seven collections in the Amicus group and nine in the Spectra group. CD34-positive cell collection efficiency was 33.20±12.23% in the Amicus group and 54.51±12.07% in the Spectra group (p<0.05), with the latter clearly superior to the former. Contamination of platelets in the products was lower in the Amicus (4.57±1.93×10
10/bag) than in the Spectra group (17.71±15.55×10
10/bag) (p<0.05).
We next modified the parameters of the Amicus program to improve collection efficiency (MNC offset: 2.1m
l, processing blood volume: 1, 800m
l×5 cycles). The number of patients was five in both groups, with seven collections in each. CD34-positive cell collection efficiency was 23.09±11.53% in the Amicus group and 50.66±15.15% in the Spectra group (p<0.05), showing that modification of parameters did not improve collection efficiency.
The advantage of the Amicus system is that it is a fully automatic type cell separator. However, the CD34-positive cell collection efficiency of Amicus was inferior to that of the Spectra manual operation program, indicating the need for further improvement of the Amicus program.
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