Abstract
Cultured malignant cells, HeLa cells, human gastric cancer cells or human lung cancer cells, were exposed to either mitomycin C or bleomycin, concentration of which were varied from 5μg/dl to 400μg/dl, for either 10, 20, or 30 minutes in vitro. As a result, mitomycin C with concentration of 200μg/dl could inhibit growth and not form colony of the malignant cells on the culture media. However bleomycin showed no inhibition of the growth and colony formation of the malignant cells under the above exposures at all.
These data should be applied to intraoperative autologous blood transfusion for a case, in whom malignant cells may migrate in the salvaged red blood cells. Namely, it was advocated that 30mg of mitomycin C should be mixed with collected red blood cells concentrated in a separating ball (Haemonetics Cell Saver®: 250ml) and should be left in place for 20 minutes for prevention of generalized dissemination of the malignant neoplasma.
It was recognized also that mitomycin C permeasted into the intracellular space of red cells and its concentration was about one fifth of that in the extracellular space after 20 minutes. However no change in deformability of erythrocyte was detected.
